Loading Plate Data in FLIMfit

Setup calibration data

  • Determine IRF shift

    • Load reference data

    • Set background, rep rate

    • Load library IRF

    • Adjust ‘IRF shift’ until the edge of the IRF sits roughly at the rising edge

    • Fit mono exponential model with free IRF shift

    • Check quality of fit

    • Note t0 value. This value should be subtracted from the IRF shift used

  • Create time varying background

    • Load media only well

    • Load camera background

    • Select central region

    • Select Tools>Export SV TVB…

Loading data

  • Load data

  • Load plate metadata

  • Load camera background images

  • Set laser repetition rate

  • Set gate maximum to camera saturation value

  • Load IRF and set IRF shift from reference measurement

  • Load time varying background

  • Save as raw dataset